4.8 Article

Reporters for sensitive and quantitative measurement of auxin response

Journal

NATURE METHODS
Volume 12, Issue 3, Pages 207-+

Publisher

NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3279

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Funding

  1. European Research Council (ERC
  2. CELLPATTERN) [281573]
  3. Netherlands Organization for Scientific Research (NWO) [ALW-820.02.019]
  4. Human Frontier Science Program (HFSP) [RGP0054-2013]
  5. Agence Nationale de la Recherche (ANR
  6. AuxiFlo) [ANR-12-BSV6-0005]
  7. Agence Nationale de la Recherche (ANR) [ANR-12-BSV6-0005] Funding Source: Agence Nationale de la Recherche (ANR)

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The visualization of hormonal signaling input and output is key to understanding how multicellular development is regulated. The plant signaling molecule auxin triggers many growth and developmental responses, but current tools lack the sensitivity or precision to visualize these. We developed a set of fluorescent reporters that allow sensitive and semiquantitative readout of auxin responses at cellular resolution in Arabidopsis thaliana. These generic tools are suitable for any transformable plant species.

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