Journal
NATURE METHODS
Volume 12, Issue 7, Pages 641-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3402
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Funding
- Ministere de l'Enseignement Superieur et de la Recherche [ANR-10-INBS-04]
- Regional Council of Aquitaine
- Singapore National Research Foundation (NRF)
- Mechanobiology Institute through an NRF grant
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Single-objective selective-plane illumination microscopy (soSPIM) is achieved with micromirrored cavities combined with a laser beam-steering unit installed on a standard inverted microscope. The illumination and detection are done through the same objective. soSPIM can be used with standard sample preparations and features high background rejection and efficient photon collection, allowing for 3D single-molecule-based super-resolution imaging of whole cells or cell aggregates. Using larger mirrors enabled us to broaden the capabilities of our system to image Drosophila embryos.
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