Journal
NATURE METHODS
Volume 12, Issue 5, Pages 411-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3319
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Funding
- Max Planck Society
- European Research Council, Deutsche Forschungsgemeinschaft (DFG) Collaborative Research Center (CRC): Molecular Principles of RNA-based Regulation [902]
- DFG CRC: Molecular and Cellular Mechanisms of Neural Homeostasis [1080]
- DFG Cluster of Excellence for Macromolecular Complexes, Goethe University, Frankfurt
- US National Institutes of Health [R01 GM062523]
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Protein synthesis is a dynamic process that tunes the cellular proteome in response to internal and external demands. Metabolic labeling approaches identify the general proteomic response but cannot visualize specific newly synthesized proteins within cells. Here we describe a technique that couples noncanonical amino acid tagging or puromycylation with the proximity ligation assay to visualize specific newly synthesized proteins and monitor their origin, redistribution and turnover in situ.
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