Journal
NATURE METHODS
Volume 12, Issue 9, Pages 845-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3479
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Funding
- Swiss National Science Foundation (SNF) [200021_134521]
- ETH Zurich [ETH-03 14-1]
- National Centre of Competence in Research (NCCR) Molecular Systems Engineering
- European Molecular Biology Organization (EMBO) [ALTF 265-2013]
- Swiss National Science Foundation (SNF) [200021_134521] Funding Source: Swiss National Science Foundation (SNF)
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Imaging native membrane receptors and testing how they interact with ligands is of fundamental interest in the life sciences but has proven remarkably difficult to accomplish. Here, we introduce an approach that uses force-distance curve-based atomic force microscopy to simultaneously image single native G protein-coupled receptors in membranes and quantify their dynamic binding strength to native and synthetic ligands. We measured kinetic and thermodynamic parameters for individual protease-activated receptor-1 (PAR1) molecules in the absence and presence of antagonists, and these measurements enabled us to describe PAR1's ligand-binding free-energy landscape with high accuracy. Our nanoscopic method opens an avenue to directly image and characterize ligand binding of native membrane receptors.
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