Journal
NATURE METHODS
Volume 12, Issue 4, Pages 339-U92Publisher
NATURE PORTFOLIO
DOI: 10.1038/NMETH.3321
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Funding
- Australian National Health and Medical Research Council (NHMRC) Australia Fellowship [631668, 631542]
- NHMRC Early Career Fellowship [APP1072662]
- EMBO Long Term Fellowship [ALTF 864-2013]
- Queensland State Government (National and International Research Alliance Program)
- EMBL Interdisciplinary Postdoc (EIPOD) under Marie Curie Actions (COFUND)
- Biotechnology and Biological Sciences Research Council [BB/J01589X/1] Funding Source: researchfish
- BBSRC [BB/J01589X/1] Funding Source: UKRI
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We compared quantitative RT-PCR (qRT-PCR), RNA-seq and capture sequencing (CaptureSeq) in terms of their ability to assemble and quantify long noncoding RNAs and novel coding exons across 20 human tissues. CaptureSeq was superior for the detection and quantification of genes with low expression, showed little technical variation and accurately measured differential expression. This approach expands and refines previous annotations and simultaneously generates an expression atlas.
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