Journal
NATURE METHODS
Volume 13, Issue 1, Pages 51-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3662
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Funding
- National Basic Research Program of China [2011CB910401]
- National Natural Science Foundation of China [81327802, 91432116]
- Science Fund for Creative Research Group of China [61421064]
- National Key Scientific Instrument & Equipment Development Program of China [2012YQ030260]
- Wuhan National Laboratory for Optoelectronics
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The reconstruction of neuronal populations, a key step in understanding neural circuits, remains a challenge in the presence of densely packed neurites. Here we achieved automatic reconstruction of neuronal populations by partially mimicking human strategies to separate individual neurons. For populations not resolvable by other methods, we obtained recall and precision rates of approximately 80%. We also demonstrate the reconstruction of 960 neurons within 3 h.
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