Journal
NATURE METHODS
Volume 12, Issue 6, Pages 515-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.3362
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Funding
- Ministry of Education, Culture, Sports, Science and Technology, Japan [23115003]
- Cooperative Research Program of 'Network Joint Research Center for Materials and Devices'
- postdoctoral fellowship from the Japan Society for the Promotion of Science
- [3306]
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Fluorescence nanoscopy has revolutionized our ability to visualize biological structures not resolvable by conventional microscopy. However, photodamage induced by intense light exposure has limited its use in live specimens. Here we describe Kohinoor, a fast-switching, positively photoswitchable fluorescent protein, and show that it has high photostability over many switching repeats. With Kohinoor, we achieved super-resolution imaging of live HeLa cells using biocompatible, ultralow laser intensity (0.004 J/cm(2)) in reversible saturable optical fluorescence transition (RESOLFT) nanoscopy.
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