Journal
NATURE METHODS
Volume 12, Issue 5, Pages 401-+Publisher
NATURE PORTFOLIO
DOI: 10.1038/nmeth.3325
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Funding
- Leona M. and Harry B. Helmsley Charitable Trust [2015PG-T1D057, 2015PG-T1D035]
- Charles H. Hood Foundation Child Health Research Award
- Glass Family Charitable Foundation
- US National Institutes of Health (NIH) [UC4 DK104218]
- NIH [1R01HD080224-01A1]
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Understanding of mammalian enhancers is limited by the lack of a technology to rapidly and thoroughly test the cell type-specific function. Here, we use a nuclease-deficient Cas9 (dCas9)-histone demethylase fusion to functionally characterize previously described and new enhancer elements for their roles in the embryonic stem cell state. Further, we distinguish the mechanism of action of dCas9-LSDSD1 at enhancers from previous dCas9-effectors.
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