4.8 Article

Light-sheet fluorescence microscopy for quantitative biology

Journal

NATURE METHODS
Volume 12, Issue 1, Pages 23-26

Publisher

NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.3219

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Funding

  1. CEF-MC by the Deutsche Forschungsgemeinschaft [EXC 115]

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In light sheet based fluorescence microscopy (LSFM), optical sectioning in the excitation process minimizes fluorophore bleaching and phototoxic effects. Because biological specimens survive long-term three-dimensional imaging at high spatiotemporal resolution, LSFM has become the tool of choice in developmental biology.

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