Magnetic Molecularly Imprinted Polymer Nanoparticles Coupled with High Performance Liquid Chromatography for Solid-Phase Extraction of Carvedilol in Serum Samples

Herein, we report a magnetic molecularly imprinted polymers (m-MIPs) using Fe3O4 as a magnetic component, carvedilol as a template molecule for the solid-phase extraction (MISPE) as the sample clean-up technique combined with high-performance liquid chromatography (HPLC) and for the controlled release of carvedilol at different pH values of 1.0 (simulated gastric fluid), 6.8 (simulated intestinal fluid), and 7.4 (simulated biological fluid). The adsorption kinetics was modeled with the pseudo-first-order and pseudosecond-order kinetics, and the adsorption isotherms were fitted with Langmuir and Freundlich models. The performance of the m-MIPs for the controlled release of carvedilol was assessed and results indicated that the magnetic MIPs also have potential applications in controlled drug release. Furthermore, the m-MIPs were applied to the extraction of carvedilol from human blood plasma samples. Carvedilol can be quantified by this method in the 2-350 mu g L-1 concentration range. The limit of detection and limit of quantification in plasma samples are 0.13 and 0.45 mu g L-1. The results from HPLC showed good precision (3.5% for 50.0 mu g L-1) and recoveries (between 85 and 93) using m-MIP from human plasma samples. (C) 2014 Wiley Periodicals, Inc.