Journal
NATURE METHODS
Volume 12, Issue 5, Pages 439-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3357
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Funding
- University of California Discovery Biotechnology Award [178517]
- US National Institutes of Health [AI065359, GM114188, EB014456]
- NanoCav, LLC.
- US National Science Foundation [CBET-1404080]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1404080] Funding Source: National Science Foundation
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We report a high-throughput platform for delivering large cargo elements into 100,000 cells in 1 min. Our biophotonic laser-assisted surgery tool (BLAST) generates an array of microcavitation bubbles that explode in response to laser pulsing, forming pores in adjacent cell membranes through which cargo is gently driven by pressurized flow. The platform delivers large items including bacteria, enzymes, antibodies and nanoparticles into diverse cell types with high efficiency and cell viability. We used this platform to explore the intracellular lifestyle of Francisella novicida and discovered that the iglC gene is unexpectedly required for intracellular replication even after phagosome escape into the cell cytosol.
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