Journal
NATURE METHODS
Volume 13, Issue 1, Pages 59-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.3667
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Funding
- US Department of Energy (DOE), Office of Science, Office of Basic Energy Sciences (OBES) [DE-AC02-76SF00515]
- Office of Science, DOE [DE-AC02-05CH11231]
- US National Institutes of Health (NIH) [P41GM103393, P41RR001209]
- Office of Science, OBES, Chemical Sciences, Geosciences, and Biosciences (CSGB) of the DOE [DE-AC02-05CH11231]
- OBES through the AMOS program within the CSGB
- DOE through the SLAC Laboratory Directed Research and Development Program
- LBNL Laboratory Directed Research and Development [DE-AC02-05CH11231]
- Stanford University Dean of Research
- Stanford ChEM-H
- SLAC National Accelerator Laboratory
- NIH [GM51266, GM082545, GM055302, GM110501, GM095887, GM102520]
- DFG-Cluster of Excellence [Sfb1078]
- Human Frontiers Science Project [RGP0005/2011, RGP0063/2013 310]
- Helmholtz Association
- NATIONAL CENTER FOR RESEARCH RESOURCES [P41RR001209] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R56GM055302, R01GM102520, R01GM055302, R01GM095887, P41GM103393, R01GM110501, P50GM082545, R01GM051266] Funding Source: NIH RePORTER
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We describe a concentric-flow electrokinetic injector for efficiently delivering microcrystals for serial femtosecond X-ray crystallography analysis that enables studies of challenging biological systems in their unadulterated mother liquor. We used the injector to analyze microcrystals of Geobacillus stearothermophilus thermolysin (2.2-angstrom structure), Thermosynechococcus elongatus photosystem II (< 3-angstrom diffraction) and Thermus thermophilus small ribosomal subunit bound to the antibiotic paromomycin at ambient temperature (3.4-angstrom structure).
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