Journal
NATURE METHODS
Volume 12, Issue 11, Pages I-IIPublisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.f.384
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Since its introduction 6 years ago, the Gibson Assembly method has been widely adopted as a preferred cloning method. Here we describe an application of Gibson Assembly beyond routine cloning: assembly in DNA library construction. Advantages of using Gibson Assembly, specifically the SGI-DNA Gibson Assembly HiFi 1 Step kit, in library construction include speed, efficiency, scarless assembly with vector and versatility.
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