Journal
JOURNAL OF APPLIED POLYMER SCIENCE
Volume 120, Issue 2, Pages 1057-1068Publisher
WILEY
DOI: 10.1002/app.33249
Keywords
hydrogels; cross-linking; biomaterials; biodegradable; poly(gamma-glutamic acid)
Categories
Funding
- National Science Council [NSC 97-2320-B-039-002-MY3, NSC 98-2815-C-039-006-E]
- Center for Inflammation Research, China Medical University, Republic of China [CMU 98-CT-12, CMU 98-N1-08]
Ask authors/readers for more resources
The process of wound healing is composed of coagulation, inflammation, fibroplasia, collagenation, epithelization, and wound contraction. The wound dressing should protect the wound from bacterial infection, maintain a moist healing environment, and promote cell migration to reconstruct damaged tissue, and be easy to apply and remove to improve patient comfort. The purpose of our study was to develop multifunctional hydrogels composed of genipin-crosslinked biodegradable biomaterials of poly(gamma-glutamic acid) and gelatin, encapsulating gentamicin to accelerate wound healing. The results of swelling ratio measurements clearly indicate that hydrogel composition of poly(gamma-glutamic acid) gelatin had a higher swelling ratio and lower peel adhesion properties than gelatin hydrogel alone. In an in vitro study, the gentamicin incorporated in prepared hydrogels effectively inhibited target microorganisms and produced a higher expression of Type I collagen in fibroblast cells. Confocal laser scanning microscopy revealed that the fibroblast cells cultured in the hydrogel membranes produced fibroblast cell migration and showed a continuous lined cytoskeletal distributing status. In the in vivo study, it was found that the gentamicin incorporated in genipin-crosslinked gamma-PGA gelatin wound dressing demonstrates the potential of such biologically functionalized dressing to accelerate wound closure and, hence, its potential clinical usefulness. (C) 2010 Wiley Periodicals, Inc. J Appl Polym Sci 120: 1057-1068, 2011
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available