N-Acetyl-D-galactosamine-Specific Lectin Isolation from Soyflour with Poly(HPMA-GMA) Beads

Soybean lectin was purified from seeds of Glycine max L.Merrill SA88. Poly(hydroxypropyl methacrylate-glycidyl methacrylate) [poly(HPMA-GMA)l beads were used as an affinity matrix and N-acetyl-D-galactosamine (GalNAc) was used as an affinity ligand. Soybean lectin adsorption with GalNAc attached poly(HPMA-GMA) beads from soybean lectin solution (in phosphate buffered saline) was 5.0 mg/g. Maximum adsorption capacity for soybean lectin from the soy flour extract was 26.0 mg/g. Elution of soybean lectin from adsorbent was accomplished by 0.5M galactose solution. Purity of soybean lectin was determined by SDS-PAGE. It was observed that soybean lectin could be repeatedly adsorbed and desorbed with GalNAc-attached poly(HPMA-GMA) beads. (C) 2008 Wiley Periodicals, Inc. J Appl Polym Sci 111: 148-154, 2009