Journal
NATURE IMMUNOLOGY
Volume 17, Issue 1, Pages 104-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/ni.3314
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Funding
- Wellcome Trust [093713/Z/10/A, 073980/Z/03/Z, 105024/Z/14/Z, 065975/Z/01/A, 097418/Z/11/Z]
- Biotechnology and Biological Sciences Research Council [BBS/E/B/000C0413, BBS/E/B/000C0415] Funding Source: researchfish
- BBSRC [BBS/E/B/000C0415, BBS/E/B/000C0413] Funding Source: UKRI
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We used high-resolution mass spectrometry to map the cytotoxic T lymphocyte (CTL) proteome and the effect of the metabolic checkpoint kinase mTORC1 on CTLs. The CTL proteome was dominated by metabolic regulators and granzymes, and mTORC1 selectively repressed and promoted expression of a subset of CTL proteins (similar to 10%). These included key CTL effector molecules, signaling proteins and a subset of metabolic enzymes. Proteomic data highlighted the potential for negative control of the production of phosphatidylinositol (3,4,5)-trisphosphate (PtdIns(3,4,5)P-3) by mTORC1 in CTLs. mTORC1 repressed PtdIns(3,4,5) P3 production and determined the requirement for mTORC2 in activation of the kinase Akt. Our unbiased proteomic analysis thus provides comprehensive understanding of CTL identity and the control of CTL function by mTORC1.
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