Journal
JOURNAL OF APPLIED PHYSIOLOGY
Volume 115, Issue 1, Pages 107-115Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/japplphysiol.00041.2013
Keywords
exercise; calcium transient; contraction; excitation-contraction coupling regulatory proteins; right ventricle
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Funding
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Fundacao de Amparo a Pesquisa do Espirito Santo
- Fundacao de Amparo a Pesquisa do Estado de Minas Gerais
- CNPq [479509/2011-5]
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We tested the effects of low-intensity endurance training (LIET) on the structural and mechanical properties of right (RV) and left ventricular (LV) myocytes. Male Wistar rats (4 mo old) were randomly divided into control (C, n = 7) and trained (T, n = 7, treadmill running at 50-60% of maximal running speed for 8 wk) groups. Isolated ventricular myocyte dimensions, contractility, Ca2+ transients {intracellular Ca2+ concentration ([Ca2+](i))}, and ventricular [Ca2+](i) regulatory proteins were measured. LIET augmented cell length (C, 152.5 +/- 2.0 mu m vs. T, 162.2 +/- 2.1 mu m; P < 0.05) and volume (C, 5,162 +/- 131 mu m(3) vs. T, 5,506 +/- 132 mu m(3); P < 0.05) in the LV but not in the RV. LIET increased cell shortening (C, 7.5 +/- 0.3% vs. T, 8.6 +/- 0.3%; P < 0.05), the [Ca2+](i) transient amplitude (C, 2.49 +/- 0.06 F/F-0 vs. T, 2.82 +/- 0.06 F/F-0; P < 0.05), the expression of sarcoplasmic reticulum Ca2+ -ATPase 2a (C, 1.07 +/- 0.13 vs. T, 1.59 +/- 0.12; P < 0.05), and the levels of phosphorylated phospholamban at serine 16 (C, 0.99 +/- 0.11 vs. T, 1.34 +/- 0.10; P < 0.05), and reduced the total phospholamban-to-sarcoplasmic reticulum Ca2+-ATPase 2a ratio (C, 1.19 +/- 0.15 vs. T, 0.40 +/- 0.16; P < 0.05) in the LV without changing such parameters in the RV. In conclusion, LIET affected the structure and improved the mechanical properties of LV but not of RV myocytes in rats, helping to characterize the functional and morphological changes that accompany the endurance training-induced cardiac remodeling.
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