Journal
JOURNAL OF APPLIED PHYSIOLOGY
Volume 114, Issue 4, Pages 453-460Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/japplphysiol.00978.2012
Keywords
sedentary; exercise; microarray; metabolism; intermittent
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Funding
- National Health and Medical Research Council (NHMRC)/Institut National de la Sante et de la Recherche Medicale (INSERM) Exchange Fellowship (NHMRC) [1440122]
- NHMRC Project Grant (NHMRC) [540107, 569940, 1000900]
- National Health and Medical Senior Principal Research Fellowship (NHMRC) [1003960]
- Victorian Public Health Research Fellowship
- Australian Research Council Future Research Fellowship [FT100100918]
- NHMRC Principal Research Fellowship (NHMRC) [526604]
- Victorian Government's Operational Infrastructure Support Program
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Latouche C, Jowett JB, Carey AL, Bertovic DA, Owen N, Dunstan DW, Kingwell BA. Effects of breaking up prolonged sitting on skeletal muscle gene expression. J Appl Physiol 114: 453-460, 2013. First published December 27, 2012; doi: 10.1152/japplphysiol.00978.2012.-Breaking up prolonged sitting has been beneficially associated with cardiometabolic risk markers in both observational and intervention studies. We aimed to define the acute transcriptional events induced in skeletal muscle by breaks in sedentary time. Overweight/obese adults participated in a randomized three-period, three-treatment crossover trial in an acute setting. The three 5-h interventions were performed in the postprandial state after a standardized test drink and included seated position with no activity and seated with 2-min bouts of light-or moderate-intensity treadmill walking every 20 min. Vastus lateralis biopsies were obtained in eight participants after each treatment, and gene expression was examined using microarrays validated with real-time quantitative PCR. There were 75 differentially expressed genes between the three conditions. Pathway analysis indicated the main biological functions affected were related to small-molecule biochemistry, cellular development, growth and proliferation, and carbohydrate metabolism. Interestingly, differentially expressed genes were also linked to cardiovascular disease. For example, relative to prolonged sitting, activity bouts increased expression of nicotamide N-methyltransferase, which modulates anti-inflammatory and antioxidative pathways and triglyceride metabolism. Activity bouts also altered expression of 10 genes involved in carbohydrate metabolism, including increased expression of dynein light chain, which may regulate translocation of the GLUT-4 glucose transporter. In addition, breaking up sedentary time reversed the effects of chronic inactivity on expression of some specific genes. This study provides insight into the muscle regulatory systems and molecular processes underlying the physiological benefits induced by interrupting prolonged sitting.
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