Journal
NATURE CHEMICAL BIOLOGY
Volume 11, Issue 3, Pages 198-U179Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nchembio.1753
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Funding
- US National Institutes of Health (NIH) [DP2OD008586]
- National Science Foundation [CBET-1151035]
- NIH [R01DA036865, R03AR061042, P30AR066527]
- American Heart Association Scientist Development Grant [10SDG3060033]
- American Heart Association Predoctoral Fellowship
- NIH Biotechnology Training Grant [T32GM008555]
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Optogenetic systems enable precise spatial and temporal control of cell behavior. We engineered a light-activated CRISPR-Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light. This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive dCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes.
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