Journal
NATURE BIOTECHNOLOGY
Volume 33, Issue 7, Pages 730-732Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.3246
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Funding
- Department of Defense MURI Award [W911NF-12-1-0420]
- US National Institutes of Health [R0IDK085714]
- China Scholarship Council
- Rio Hortega Fellowship
- Martin Escudero Fellowship
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MicroRNAs (miRNAs) have emerged as promising diagnostic biomarkers. We introduce a kinetic fingerprinting approach called single-molecule recognition through equilibrium Poisson sampling (SiMREPS) for the amplification-free counting of single unlabeled miRNA molecules, which circumvents thermodynamic limits of specificity and virtually eliminates false positives. We demonstrate high-confidence, single-molecule detection of synthetic and endogenous miRNAs in both buffer and minimally treated biofluids, as well as >500-fold discrimination between single nucleotide polymorphisms.
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