Journal
NATURE BIOTECHNOLOGY
Volume 33, Issue 11, Pages 1162-+Publisher
NATURE RESEARCH
DOI: 10.1038/nbt.3389
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Funding
- Institute for Basic Science [IBS-R021-D1]
- Next-Generation BioGreen21 Program [PJ01104501, PJ01104502]
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Editing plant genomes without introducing foreign DNA into cells may alleviate regulatory concerns related to genetically modified plants. We transfected preassembled complexes of purified Cas9 protein and guide RNA into plant protoplasts of Arabidopsis thaliana, tobacco, lettuce and rice and achieved targeted mutagenesis in regenerated plants at frequencies of up to 46%. The targeted sites contained germline-transmissible small insertions or deletions that are indistinguishable from naturally occurring genetic variation.
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