Journal
NATURE BIOTECHNOLOGY
Volume 33, Issue 8, Pages 842-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.3280
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Funding
- Novo Nordisk Foundation
- Kirsten og Freddy Johansen Fonden
- Mizutani Foundation
- A.P. Moller og Hustru Chastine Mc-Kinney Mollers Fond til Almene Formaal
- Copenhagen University Excellence Programme for Interdisciplinary Research [CDO2016]
- Danish National Research Foundation [DNRF107]
- Danish Councils for Strategic and Independent Research
- Lundbeck Foundation [R108-2012-10312] Funding Source: researchfish
- Novo Nordisk Fonden [NNF10CC1016517] Funding Source: researchfish
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Production of glycoprotein therapeutics in Chinese hamster ovary (CHO) cells is limited by the cells' generic capacity for N-glycosylation, and production of glycoproteins with desirable homogeneous glycoforms remains a challenge. We conducted a comprehensive knockout screen of glycosyltransferase genes controlling N-glycosylation in CHO cells and constructed a design matrix that facilitates the generation of desired glycosylation, such as human-like alpha 2,6-linked sialic acid capping. This engineering approach will aid the production of glycoproteins with improved properties and therapeutic potential.
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