Journal
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
Volume 59, Issue 8, Pages 5088-5091Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.00420-15
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Funding
- Centre of Research Excellence [G1001021]
- National Health and Medical Research Council of Australia [G1046886, PF1002076]
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Comparison of green fluorescent protein expression from outward-facing promoters (P-OUT) of ISAba1, ISEcp1, and ISAba125 revealed approximate equivalence in strength, intermediate between PCS (strong) and PCWTGN-10 (weak) class 1 integron promoter variants, >30-fold stronger than P-OUT of ISCR1, and >5 times stronger than Ptac. Consistent with its usual role, PCWTGN-10 produces more mRNA from a downstream gfp gene transcriptionally linked to a usual PCWTGN-10-associated gene cassette than does P-OUT of ISAba1.
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