Journal
NATURE
Volume 518, Issue 7539, Pages 376-380Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nature14229
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Funding
- Boehringer Ingelheim fellowship
- Elite Network of Bavaria
- Genome Canada
- Genome British Columbia Science and Technology Innovation Centre
- Natural Sciences and Engineering Research Council of Canada
- LMUexcellent initiative
- Bavarian Research Center of Molecular Biosystems
- Deutsche Forschungsgemeinschaft [GRK1721, SFB646]
- European Research Council Advanced Grant TRANSIT
- Jung-Stiftung
- Volkswagen Foundation
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The conserved co-activator complex Mediator enables regulated transcription irntiation by RNA polymerase (Pol) II. Here we reconstitute an active 15-subunit core Mediator (cMed) comprising all essential Mediator subunits from Saccharomyces erevisiae. The cryo-electron microscopic structure of cMed bound to a core initiation complex was determined at 9.7 A resolution. cMed binds Pol II around the Rpb4-Rpb7 stalk near the carboxy-terminal domain (CTD). The Mediator head module binds the Pol II dock and the TFIIB ribbon and stabilizes the initiation complex. The Mediator middle module extends to the Fol II foot with a 'plank' that may influence polymerase conformation. The Mediator subunit Medl4 forms a 'beam' between the head and middle modules and connects to the tail module that is predicted to bind transcription activators located on upstream DNA. The Mediator 'arm' and 'hook' domains contribute to a 'cradle' that may position the CTD and TFIIH kinase to stimulate Pol II phosphorylation.
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