Journal
JOURNAL OF APPLIED PHYCOLOGY
Volume 27, Issue 1, Pages 545-553Publisher
SPRINGER
DOI: 10.1007/s10811-014-0293-7
Keywords
Fucus evanescens; Fucose-containing sulfate polysaccharides; Fucoidan; Polyphenols; Antioxidant activity; Extraction method
Funding
- Russian Foundation for Basic Research [12-04-0069, 14-04-00973]
- RAS Program on Molecular and Cell Biology
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The fucose-containing sulfated polysaccharides (FCSPs) from Fucus evanescens were obtained via different procedures: by hot acidic extraction and with aqueous calcium chloride solution, followed by separation FCSPs using anion-exchanging chromatography. Chemical characteristics of these polysaccharide fractions were determined. It was shown that extraction with aqueous calcium chloride solution provides a purified preparation of the FCSPs. The crude polysaccharide and its chromatography purified fractions obtained by this method have significantly lower contents of laminaran, uronic acids, and polyphenols in comparison with FCSPs obtained by hot acid extraction. All polysaccharides exhibited total antioxidant capacity and 1,1-diphenyl-2-pycrilhydrazyl (DPPH) radical-scavenging activity and low 2,2'-azino-bis 3-ethyl benzothiazoline-6-sulfuric acid (ABTS) radical-scavenging activity. A strong positive correlation between polyphenol content in FCSPs and their antioxidant activity was found. This fact may indicate that phenols play a key role in the previously described antioxidant capacity of FCSPs.
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