Journal
JOURNAL OF APPLIED PHYCOLOGY
Volume 24, Issue 5, Pages 1169-1176Publisher
SPRINGER
DOI: 10.1007/s10811-011-9746-4
Keywords
Chlamydomonas sp ICE-L; Lipid content; BODIPY505/515; Rapid estimation
Funding
- National Natural Science Foundation of China [40876102, 40876107, 41176153]
- Shandong Science and Technology plan project [2011GHY11528]
- National special fund for transgenic project [2009ZX08009-019B]
- Hi-Tech Research and Development Program (863) of China [2009AA10Z106]
- Natural Science Foundation of Shandong Province [2009ZRA02075]
- Qingdao Municipal Science and Technology plan project [09-2-5-8-hy, 10-4-1-13-hy]
- National Marine Public Welfare Research Project [200805069]
- National Science & Technology Pillar Program [2008BAD95B11]
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Chlamydomonas sp. ICE-L, isolated from Antarctic coastal marine environments, was selected as a high lipid producer, which may be useful for biodiesel production. The lipophilic fluorescent dye BODIPY505/515 was used to determine the algal lipid content. Lipid bodies stained with BODIPY505/515 have a characteristic green fluorescence, and their volumes were determined using the sphere volume formula. In this study, lipid accumulation by Chlamydomonas ICE-L was analyzed under different cultivation conditions (nitrogen deficiency and UV-B radiation). The results demonstrated that nitrogen deficiency and UV-B radiation could significantly promote the accumulation of lipid content per cell. The highest yields of total lipid content (reaching 84 mu L L-1) were obtained in full Provasoli medium after 12 days of cultivation, but not in the nitrogen-deficient medium. The inoculum used in this experiment was obtained from the late-exponential growth phase. The main reason was that the cell numbers in nitrogen-deficient medium had not increased and total lipid contents were offset by the lower growth rate. Considering the high lipid content in Chlamydomonas sp. ICE-L, this alga might be a promising alternative species for production of microalgal oil for the production of renewable biodiesel in the future.
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