Journal
JOURNAL OF APPLIED PHYCOLOGY
Volume 23, Issue 6, Pages 1053-1057Publisher
SPRINGER
DOI: 10.1007/s10811-010-9623-6
Keywords
Cytometry; Fluorescence-activated cell sorting (FACS); Nile Red (NR); Neutral lipids; Tetraselmis suecica
Funding
- Consorcio Estrategico Nacional de Investigacion Tecnica (CENIT)-Proyecto de Investigacion para el Impulso del Biodiesel en Espana (PIIBE), of the Spanish Government
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Biodiesel from algae is considered an alternative for a third generation of biofuels. However, most microalgae are not lipogenic during fast growth periods, but high-lipid content occurs at resting stages. Microalgae biomass production for biodiesel needs continuous high volumetric and aerial yields and large amount of neutral lipid in the biomass. These requirements are similar to demanding a marathon runner to be obese. We show that by using cell sorting capabilities of flow cytometers, in combination with the lipid-soluble fluorescent dye Nile Red, we can isolate and select cells with a high and stable lipid content. In our study, we were able to select the equivalent of a stable fat marathon runner through three sorting events obtained from wild populations of Tetraselmis suecica.
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