Journal
JOURNAL OF APPLIED PHYCOLOGY
Volume 22, Issue 5, Pages 645-649Publisher
SPRINGER
DOI: 10.1007/s10811-010-9505-y
Keywords
beta-carotene; Carotenoids; Dunaliella salina; Fluorescence microscopy; Globules
Funding
- Technology foundation STW, The Netherlands Organisation for Scientific Research [WLM.6622]
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Dunaliella salina is a halotolerant green alga that is well known for its carotenoid producing capacity. The produced carotenoids are mainly stored in lipid globules. For various research purposes, such as production and extraction kinetics, we would like to determine and/or localise the carotenoid globules in vivo. In this study, we show that the carotenoid-rich globules emit clear green fluorescence, which can be used in, for example, fluorescence microscopy (e.g. CLSM) to obtain pictures of the cells and their carotenoid content.
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