Journal
JOURNAL OF APPLIED PHYCOLOGY
Volume 21, Issue 6, Pages 657-668Publisher
SPRINGER
DOI: 10.1007/s10811-008-9399-0
Keywords
Locked nucleic acids; Oligonucleotide probes; Microalgae; Sandwich hybridisation; Microarray
Funding
- EU [GOCE-CT-2003-505491, COOP-CT-2004-508435-ALGADEC]
- Alfred Wegener Institute for Polar and Marine Research
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Biosensors and microarrays are powerful tools for species detection and monitoring of microorganisms. A reliable identification of microorganisms with probe-based methods requires highly specific and sensitive probes. The introduction of locked nucleic acid (LNA) promises an enhancement of specificity and sensitivity of molecular probes. In this study, we compared specificity and sensitivity of conventional probes and LNA modified probes in two different solid phase hybridisation methods: sandwich hybridisation on biosensors and on DNA microarrays. In combination with DNA-microarrays, the LNA probes displayed an enhancement of sensitivity, but also gave more false-positive signals. With the biosensor, the LNA probes showed neither signal enhancement nor discrimination of a single mismatch. In all cases, conventional DNA probes showed equal or better results than LNA probes. In conclusion, LNA technology may have great potential in methods that use probes in suspension and in gene expressions studies, but under certain solid surface-hybridisation applications, they do not improve signal intensity.
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