Journal
JOURNAL OF APPLIED PHYCOLOGY
Volume 22, Issue 1, Pages 25-31Publisher
SPRINGER
DOI: 10.1007/s10811-009-9420-2
Keywords
Porphyra yezoensis; R-phycoerythrin; Hydrophobic interaction chromatography; Expanded bed absorption; Ion-exchange chromatography; Streamline column
Funding
- 863 Project of China [2007AA09Z406]
- National Natural Science Foundation of China [40806063, 30830015]
- National Development [2006BAD09A04]
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R-phycoerythrin was purified by means of phenyl-sepharose expanded bed absorption and DEAE-sepharose ion-exchange chromatography from Porphyra yezoensis, one of the largest and important aquaculture species in China. Final R-phycoerythrin preparation was characterized by purity ratio above 4 and the homogeneity in native PAGE, respectively. The results of absorption spectrum, fluorescence spectrum and SDS-PAGE were in agreement with previous reports on R-PE. The yield of R-phycoerythrin was 0.82 mg g(-1) wet leafy gametophyte of P. yezoensis. This method is a high-protein recovery technology while reducing processing time, and is suitable for the large batch production of R-phycoerythrin, which will enhance the value of P. yezoensis in China, especially the inferior P. yezoensis which can not be used for flake processing.
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