Journal
JOURNAL OF APPLIED MICROBIOLOGY
Volume 108, Issue 3, Pages 908-916Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1365-2672.2009.04485.x
Keywords
antagonist; bacteriocin; biocontrol; probiotics; Staphylococcus hominis MBBL 2-9
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Funding
- BioGreen 21 Program, Rural Development Administration, Republic of Korea [20080401034009]
- Korean Government [KRF-2008-005-J00202]
- Rural Development Administration (RDA), Republic of Korea [20080401034009] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Aims: To isolate and characterize an antagonist for use as probiotic agent in the biocontrol of Staphylococcus aureus. Methods and Results: Bacteria that exhibited antimicrobial activity against Gram-positive bacteria including Staph. aureus were isolated from 12 healthy women, with Staphylococcus hominis MBBL 2-9 showing the strongest activity. The bacteriocin produced by Staph. hominis MBBL 2-9 was purified by 60% ammonium sulfate saturation, ultrafiltration, HLB cartridge and reverse-phase HPLC. The molecular weight was estimated as 2038 center dot 2 Da by MALDI-TOF mass spectrometry. The antagonist survived up to 2 h in artificial gastric juice (pH 2 center dot 5) and grew in the presence of 1% porcine bile extract. In addition, Staph. hominis MBBL 2-9 adhered effectively to HT-29 epithelial cell line. Conclusion: Staphylococcus hominis MBBL 2-9 exhibited desirable probiotic traits such as acid tolerance, bile resistance and adherence to epithelial cell line. The bacterium also produced a bacteriocin with unique molecular weight and high antimicrobial activity similar to traditional antibiotics. Significance and Impact of the Study: This study is the first report of a bacteriocin-producing Staph. hominis MBBL 2-9 that has potential for use as a probiotic agent against Staph. aureus.
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