Journal
JOURNAL OF APPLIED MICROBIOLOGY
Volume 107, Issue 1, Pages 136-144Publisher
WILEY
DOI: 10.1111/j.1365-2672.2009.04185.x
Keywords
hyaluronan synthase; hyaluronic acid; polymer size; regulation mechanism; UDP-glucose-6-dehydrogenase
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To determine the effects of the ratios of hyaluronan synthase expression level to precursor sugar UDP-GlcA biosynthesis ability on the molecular weight (MW) of hyaluronic acid (HA) in recombinant Lactococcus lactis. The genes szHasA (hyaluronan synthase gene) and szHasB (UDP-glucose-6-dehydrogenase gene) of Streptococcus zooepidemicus were introduced into L. lactis under the control of nisA promoter and lacA promoter respectively, resulting in a dual-plasmid controlled expression system. The effects of the ratios of hyaluronan synthase expression level to the precursor sugar UDP-GlcA biosynthesis ability under different induction concentration collocations with nisin and lactose on the MW of HA in recombinant L. lactis were determined. The results showed that the final weight-average molecular weight (MW) of HA correlated with the relative ratios of HasA (hyaluronan synthase) expression level to the concentration of UDP-GlcA. Regulating the relative ratios of HasA expression level to the precursor sugar biosynthesis ability was an efficient method to control the size of HA. This study put forward a guide to establish an efficacious way to control the size of HA in fermentation.
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