Journal
JOURNAL OF APPLIED MICROBIOLOGY
Volume 106, Issue 3, Pages 952-966Publisher
WILEY
DOI: 10.1111/j.1365-2672.2008.04064.x
Keywords
16S rDNA; anaerobic digestion; biogas digester; denaturing gradient gel electrophoresis (DGGE); microbial community; pig manure
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Funding
- Chinese Academy of Sciences [KSCX2-YW-G-022]
- National High Technology Research and Development Key Program of China [2007AA021302]
- National Natural Science Foundation of China [30700017]
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To identify the bacterial and archaeal composition in a mesophilic biogas digester treating pig manure and to compare the consistency of two 16S rDNA-based methods to investigate the microbial structure. Sixty-nine bacterial operational taxonomic units (OTU) and 25 archaeal OTU were identified by sequencing two 16S rDNA clone libraries. Most bacterial OTU were identified as phyla of Firmicutes (47.2% of total clones), Bacteroides (35.4%) and Spirochaetes (13.2%). Methanoculleus bourgensis (29.0%), Methanosarcina barkeri (27.4%) and Methanospirillum hungatei (10.8%) were the dominant methanogens. Only 9% of bacterial and 20% of archaeal OTU matched cultured isolates at a similarity index of >= 97%. About 78% of the dominant bacterial (with abundance > 3%) and 83% of archaeal OTU were recovered from the denaturing gradient gel electrophoresis (DGGE) bands of V3 regions in 16S rDNAs. In the digester, most bacterial and archaeal species were uncultured; bacteria belonging to Firmicutes, Bacteroides and Spirochaetes seem to take charge of cellulolysis, proteolysis, acidogenesis, sulfur-reducing and homoacetogenesis; the most methanogens were typical hydrogenotrophic or hydrogenotrophic/aceticlastic; DGGE profiles reflected the dominant microbiota. This study gave a first insight of the overall microbial structure in a rural biogas digester and also indicated DGGE was useful in displaying its dominant microbiota.
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