Journal
JOURNAL OF APPLIED MICROBIOLOGY
Volume 105, Issue 6, Pages 1982-1990Publisher
WILEY
DOI: 10.1111/j.1365-2672.2008.03958.x
Keywords
bacteriocin; lactic acid bacteria; Lactococcus lactis; lantibiotic; nisin
Categories
Funding
- Ministry of Economy, Trade and Industry of Japan
- Japan Society for the Promotion of Science (JSPS)
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To characterize the genetic and biochemical features of nisin Q. The nisin Q gene cluster was sequenced, and 11 putative orfs having 82% homology with the nisin A biosynthesis gene cluster were identified. Nisin Q production was confirmed from the nisQ-introduced nisin Z producer. In the reporter assay, nisin Q exhibited an induction level that was threefold lower than that of nisin A. Nisin Q demonstrated an antimicrobial spectrum similar to those of the other nisins. Under oxidizing conditions, nisin Q retained a higher level of activity than nisin A. This higher oxidative tolerance could be attributed to the presence of only one methionine residue in nisin Q, in contrast to other nisins that contain two. The 11 orfs of the nisin producers were identical with regard to their functions. The antimicrobial spectra of the three natural nisins were similar. Nisin Q demonstrated higher oxidative tolerance than nisin A. Genetic and biochemical features of nisin Q are similar to those of other variants. Moreover, owing to its higher oxidative tolerance, nisin Q is a potential alternative for nisin A.
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