Journal
JOURNAL OF APPLIED MICROBIOLOGY
Volume 105, Issue 6, Pages 1835-1850Publisher
WILEY
DOI: 10.1111/j.1365-2672.2008.03949.x
Keywords
anaerobic digestion; archaea; biogas plant; degradation; mcrA clone library; methanogens; RFLP analysis; 16S rDNA clone library
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Funding
- German Federal Ministry of Food, Agriculture and Consumer Protection BMELV)/Agency of Renewable Resources [22011804]
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The Archaea diversity was evaluated in an agricultural biogas plant supplied with cattle liquid manure and maize silage under mesophilic conditions. Two different genes (16S rRNA; methyl-coenzyme-M-reductase, MCR) targeted by three different PCR primer sets were selected and used for the construction of three clone libraries comprising between 104 and 118 clones. The clone libraries were analysed by restriction fragment polymorphism (RFLP). Between 11 and 31 operational taxonomic units (OTUs) were detected and assigned to orders Methanomicrobiales, Methanosarcinales and Methanobacteriales. Over 70% of all Archaea OTUs belong to the order Methanomicrobiales which mostly include hydrogenotrophic methanogens. Acetotrophic methanogens were detected in minor rates. Similar relative values were obtained by a quantitative real-time PCR analysis. The results implied that in this biogas plant the most of the methane formation resulted from the conversion of H-2 and CO2. This study reports, for the first time, a molecular analysis of the archaeal community in this type of agricultural biogas plants. Therein the hydrogenotrophic methanogenesis seems to be the major pathway of methane formation. These results are in contrast with the common thesis that in biogas fermentations the primary substrate for methanogenesis is acetate.
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