Effect of various environmental factors on the viability of Cryptosporidium parvum oocysts

Aims: To evaluate individual and combined effects of temperature (4, 18 and 25 degrees C), pH (7 and 10), ammonia (5 and 50 mg l(-1)) and exposure time (1, 2, 4 and 6 days) on the viability of Cryptosporidium parvum oocysts in water. Methods and Results: The viability of oocysts was evaluated using the fluorogenic vital dyes assay (4',6-diamidino-2-phenylindole and propidium iodide). All the factors analysed (temperature, pH, ammonia and exposure time) and their interaction were statistically significant (P < 0.005). Exposure of oocysts to pH 10 for 6 days at 25 degrees C reduced oocyst viability from similar to 80% to 51%. Similarly, the exposure of C. parvum oocysts to 5 mg NH3 l(-1) and 50 mg NH3 l(-1) for 4 days reduced their viability from between similar to 80% to 41.5% and 14.8%, respectively. Conclusions: The interaction between pH, temperature and exposure time may have adverse effects on the survival of C. parvum oocysts in water. Low concentrations of ammonia, as commonly found in alga-based wastewater systems, over a long period of time can produce high C. parvum oocyst inactivation rates. Significance and Impact of the Study: This study provides relevant data on the inactivation of C. parvum oocysts in alga-based wastewater-treatment systems in the northwest of Spain.