Journal
JOURNAL OF APPLIED MICROBIOLOGY
Volume 105, Issue 6, Pages 1768-1776Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1365-2672.2008.03868.x
Keywords
Agrobacterium tumefaciens; dhaB; glycerol; 1; 3-propanediol; Saccharomyces cerevisiae; yqhD
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Saccharomyces cerevisiae is a safe micro-organism used in fermentation industry. 1,3-Propanediol is an important chemical widely used in polymer production, but its availability is being restricted owing to its expensively chemical synthesis. The aim of this study is to engineer a S. cerevisiae strain that can produce 1,3-propanediol at low cost. By using d-glucose as a feedstock, S. cerevisiae could produce glycerol, but not 1,3-propanediol. In this study, we have cloned two genes yqhD and dhaB required for the production of 1,3-propanediol from glycerol, and integrated them into the chromosome of S. cerevisiae W303-1A by Agrobacterium tumefaciens-mediated transformation. Both genes yqhD and dhaB functioned in the engineered S. cerevisiae and led to the production of 1,3-propanediol from d-glucose. Saccharomyces cerevisiae can be engineered to produce 1,3-propanediol from low-cost feedstock d-glucose. To our knowledge, this is the first report on developing S. cerevisiae to produce 1,3-propanediol by using A. tumefaciens-mediated transformation. This study might lead to a safe and cost-efficient method for industrial production of 1,3-propanediol.
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