Modifications to the IMAGEJ computer assisted sperm analysis plugin greatly improve efficiency and fundamentally alter the scope of attainable data

Computer assisted sperm analysis (CASA) has greatly enhanced the quality and quantity of data that can be collected on spermatozoa movements. Open source imagej software allows any researcher to have access to such analyses, but CASA systems in general are not designed to ably process large numbers of video sequences. Here we introduce a modified imagej CASA_automated plugin that fundamentally enhances efficiency and will allow higher quality research to be conducted with no extra post-microscope effort.