Journal
JOURNAL OF APPLIED GENETICS
Volume 55, Issue 3, Pages 307-312Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s13353-014-0211-3
Keywords
Hordeum chilense; Start Codon Targeted (SCoT) markers; Triticum turgidum L. ssp durum; Tritordeum
Funding
- International Consortium HY-WHEAT - COMPETE program (Programa Operacional Factores de Competitividade) [P-KBBE/AGR-GPL/0002/2010]
- Quadro de Referencia Estrategico Nacional (QREN)
- European Union (Fundo Europeu de Desenvolvimento Regional)
- Portuguese Foundation for Science and Technology (FCT)
- Fundação para a Ciência e a Tecnologia [P-KBBE/AGR-GPL/0002/2010] Funding Source: FCT
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Hexaploid tritordeum (H(ch)H(ch)AABB; 2n = 42) results from the cross between Hordeum chilense ((HHch)-H-ch; 2n = 14) and cultivated durum wheat (Triticum turgidum ssp. durum (AABB; 2n = 28). Morphologically, tritordeum resembles the wheat parent, showing promise for agriculture and wheat breeding. Start Codon Targeted (SCoT) polymorphism is a recently developed technique that generates gene-targeted markers. Thus, we considered it interesting to evaluate its potential for the DNA fingerprinting of newly synthesized hexaploid tritordeums and their respective parents. In this study, 60 SCoT primers were tested, and 18 and 19 of them revealed SCoT polymorphisms in the newly synthesized tritordeum lines HT27 and HT22, respectively, and their parents. An analysis of the presence/absence of bands among tritordeums and their parents revealed three types of polymorphic markers: (i) shared by tritordeums and one of their parents, (ii) exclusively amplified in tritordeums, and (iii) exclusively amplified in the parents. No polymorphism was detected among individuals of each parental species. Three SCoT markers were exclusively amplified in tritordeums of lines HT22 and HT27, being considered as polyploidization-induced rearrangements. About 70 % of the SCoT markers of H. chilense origin were not transmitted to the allopolyploids of both lines, and most of the SCoTs scored in the newly synthesized allopolyploids originated from wheat, reinforcing the potential use of tritordeum as an alternative crop.
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