Journal
JOURNAL OF APPLIED GENETICS
Volume 49, Issue 4, Pages 357-366Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/BF03195634
Keywords
animal cytogenetics; chromosome banding; FISH; techniques
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This paper describes the most common cytogenetic techniques we routinely adopt in our laboratories for producing high-resolution banding on prometaphase stage chromosomes, from synchronized or nonsynchronized blood cultures. Special emphasis is given to the FISH procedures applied to prometaphase chromosomes for mapping purposes. Each section includes historical information, basic principles for the given technique. its primary Use ill veterinary cytogenetics, and major limitations. Supplementary material (protocols and chemicals used) are available on our website. Even though these techniques mainly refer to the Bovidae, they call be easily extended and adapted to members of other taxa.
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