Journal
JOURNAL OF APPLIED CRYSTALLOGRAPHY
Volume 46, Issue -, Pages 1481-1489Publisher
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S0021889813022358
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Funding
- Fight For Sight Project [1360]
- Science and Technology Facilities Council [MX7062]
- National Institute for Health Research (NIHR) Biomedical Research Centre based at Moorfields Eye Hospital NHS Foundation Trust
- UCL Institute of Ophthalmology
- Fight for Sight [1359/60] Funding Source: researchfish
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A quantitative map of collagen fibril orientation across the human eyeball coat, including both the cornea and the sclera, has been obtained using a combination of synchrotron wide-angle X-ray scattering (WAXS) and three-dimensional point mapping. A macromolecular crystallography beamline, in a custom-modified fibre diffraction setup, was used to record the 1.6 nm intermolecular equatorial reflection from fibrillar collagen at 0.5 mm spatial resolution across a flat-mounted human eyeball coat. Fibril orientation, derived as an average measure of the tissue thickness, was quantified by extraction of the azimuthal distribution of WAXS scatter intensity. Vector plots of preferential fibre orientation were remapped onto an idealized eyeball surface using a custom-built numerical algorithm, to obtain a three-dimensional representation of the collagen fibril architecture. (C) 2013 International Union of Crystallography Printed in Singapore - all rights reserved
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