Journal
NATURAL PRODUCT RESEARCH
Volume 29, Issue 19, Pages 1798-1803Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/14786419.2015.1007137
Keywords
Paris polyphylla; separation; HPLC-ESI-QTOF-MS; MS
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Funding
- National Natural Science Foundation of China [31460073, 31260044]
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The root of Paris polyphylla (RPP) is widely used as a traditional Chinese medicine for a long time due to the good properties of heat-clearing and detoxicating, detumescence, sedation, acesodyne and haemostasis. To clarify on the bioactive substances and ensure the safety in clinical medication, a feasible and accurate strategy was developed by applying the high-performance liquid chromatography coupled to electrospray ionisation and quadrupole time-of-flight-mass spectrometry (HPLC-ESI-QTOF-MS/MS). Separation was performed an Agilent poroshell 120 EC-C18 column (2.7x100mm, i.d., 2.7m) with 0.1% formic acid aqueous solution and acetonitrile as the mobile phase under gradient conditions. Based on the proposed strategy, 30 constituents, mainly including steroidal saponins, were characterised or tentatively identified, 2 of which were the first to be reported as the potential new steroidal saponins in RPP. In conclusion, the HPLC-ESI-QTOF-MS/MS is a feasible and credible technique to separate and identify steroidal saponins from botanical extracts.
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