Journal
JOURNAL OF ANTIBIOTICS
Volume 66, Issue 12, Pages 691-699Publisher
JAPAN ANTIBIOTICS RESEARCH ASSOC
DOI: 10.1038/ja.2013.76
Keywords
biosynthesis; enzyme; gene cluster; beta-glutamate-beta-decarboxylase; macrolactam; polyketide; Streptomyces
Funding
- the Ministry of Education, Culture, Sports, Science and Technology
- Nagase Science and Technology Foundation
- Takeda Science Foundation
- Grants-in-Aid for Scientific Research [22108003] Funding Source: KAKEN
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A biosynthetic gene cluster for the 24-membered macrolactam antibiotic incednine was identified from the producer strain, Streptomyces sp. ML694-90F3. Among the putative incednine biosynthetic enzymes, a novel pyridoxal 5'-phosphate (PLP)-dependent beta-glutamate-beta-decarboxylase, IdnL3, was functionally characterized in vitro by demonstrating its (S)-3-aminobutyrate- forming activity with beta-glutamate in the presence of PLP. Because (S)-3-aminobutyrate is known for the direct precursor of incednine, this enzyme supplies the unique beta-amino acid starter unit. The identified gene cluster encodes five characteristic b-amino acid carrying enzymes, consisting of a pathway-specific ATP-dependent ligase, a discrete acyl carrier protein (ACP), beta-aminoacyl-ACP b-amino group-protecting ATP-dependent ligase, dipeptidyl-ACP: PKS-loading ACP dipeptidyltransferase and a terminal amino acid peptidase, which are completely conserved in beta-amino acid-containing macrolactam biosynthetic gene clusters. Overall, a plausible biosynthetic pathway for incednine was proposed.
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