Journal
JOURNAL OF ANTIBIOTICS
Volume 62, Issue 6, Pages 309-313Publisher
JAPAN ANTIBIOTICS RESEARCH ASSOC
DOI: 10.1038/ja.2009.33
Keywords
antibiotic production; nsdA; sporulation; Streptomyces bingchengensis
Funding
- National Key Technology RD Program [2006BAD31B02]
- Program for New Century Excellent Talents in University
- Outstanding Youth Foundation of Heilongjiang Province [JC200706]
- China Postdoctoral Science Foundation [20070410274]
Ask authors/readers for more resources
To investigate the function of nsdA in Streptomyces bingchengensis, it was cloned and sequenced, which presented an 89.89% identity with that of S. coelicolor. The lambda RED-mediated PCR-targeting technique was used to create nsdA replacement in the S. bingchengensis_226541 chromosome. The nsdA disruption mutant, BC29, was obtained, which produced more pigment and spores than did the ancestral strain. HPLC analysis revealed that the disruption of nsdA efficiently increased milbemycin A(4) production and nanchangmycin production by 1.5-fold and 9-fold, respectively. Complementation of the nsdA mutation restored the phenotype and antibiotic production. These results showed that nsdA negatively affected sporulation and antibiotic production in S. bingchengensis. The Journal of Antibiotics (2009) 62, 309-313; doi:10.1038/ja.2009.33; published online 15 May 2009
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available