Journal
NANOTOXICOLOGY
Volume 10, Issue 3, Pages 303-311Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/17435390.2015.1054909
Keywords
Biomolecular corona; coelomocytes; gene expression; NM-300K; toll-like receptors
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Funding
- EU FP7-project MARINA [263215]
- Danish Strategic Research Council (SIDANA) [09-067185]
- Danish Research Council (FUU) [1-5971-10000377]
- Nordic Council of Ministers
- NKG environmental nanoproject
- Medical Faculty Research Foundation
- University of Pecs (PTE AOK-KA) [2013/09]
- Janos Bolyai Research Foundation of the Hungarian Academy of Sciences
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Previously we have identified lysenin as a key protein constituent of the secretome from Eisenia fetida coelomocytes and revealed its critical importance in priming interactions between the cells and the protein corona around nanosilver. As alterations of the protein environment can directly affect the corona composition, the extent to which nanoparticles influence the cells' protein secretion profile is of remarkable interest that has rarely acquired attention. Here, we have probed transcriptional responses of E. fetida coelomocytes to the representative nanosilver NM-300K (15nm) in a time-dependent manner (2, 4, 8 and 24h at a low-cytotoxic concentration), and examined the implication of the temporal changes in transcriptional profiles of secretory proteins with a particular reference to that of lysenin. NM-300K was accumulated in/at the cells and lysenin was, after transient induction, gradually suppressed over time indicating a negative feedback cycle. This may limit further enrichment of lysenin in the corona and thereby decrease the lysenin-assisted uptake of the nanoparticles. Other differentially expressed genes were those involved in metal stress (likewise in AgNO3-stressed cells) and in Toll-like receptor (TLR) signaling. This offers an intriguing perspective of the nanosilver pathophysiology in earthworms, in which the conserved pattern recognition receptor TLRs may play an effector role.
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