Journal
JOURNAL OF ANALYTICAL CHEMISTRY
Volume 68, Issue 11, Pages 1007-1013Publisher
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S1061934813110087
Keywords
second-derivative spectrophotometry; vitamin B-3; cosmetic preparations; TLC; HPLC
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Differential spectrophotometry and liquid chromatography (TLC, HPLC) were used to qualitative and quantitative determination of nicotinamide (vitamin B-3) in commercial cosmetics creams. The liquid-liquid extraction has been used as pretreatment processes for cosmetics samples prior to their analyses. Differential spectra were collected using a UV-Vis spectrophotometer with the range of 220-350 nm, in a 1-cm layer, with the speed of spectrum registering at 240 nm/min, the spectra width of the radiation leaving the monochromator of 1.00 nm and the increase in wavelength of 1 nm. The measuring methods of peak-zero and peak-peak were used. The reversed-phase determination of vitamin B-3 was conducted on a LiChrospher 100 RP-18 column (250 x 4 mm, 10 mu m) at 260 nm, using a mixture of KH2PO4 (0.02 M)-methanol (9: 1) as a mobile phase (the flow rate - 1.5 mL/min). The UV method (D-2) for the determination of vitamin B-3 in cosmetic products is accurate with the comparison of reference method of HPLC.
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