Journal
JOURNAL OF ANALYTICAL CHEMISTRY
Volume 67, Issue 4, Pages 364-369Publisher
PLEIADES PUBLISHING INC
DOI: 10.1134/S1061934812020050
Keywords
antioxidant activity; potentiometry; chronoamperometry; mediator system
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Funding
- Russian Foundation for Basic Research [07-03-96071-p_ural_a]
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A novel version of a chronoamperometric method of antioxidant determination is described. It is based on the use of the oxidation current of ferrocyanide formed in the reaction of antioxidants to be determined with potassium ferricyanide as an analytical signal. So the total concentration of all antioxidants present in the analyzed solution, i.e., total antioxidant capacity, is measured. The results of blood plasma analysis by the newly presented and certified potentiometry method are in good agreement with each other, R (2) = 0.9980. The detection limit makes 2 x 10(-6) M for uric and ascorbic acids and 5 x 10(-6) M for cysteine and glutathione.
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