Chromatographic determination of some tropane alkaloids in Datura metel

An analytical scenario has been developed for the determination of some tropane alkaloids in Datura metel, including sample preparation by solid-phase extraction and chromatographic (GC and HPLC) determination of the analytes. The proposed scenario is rapid and provides the complete recovery of alkaloids; the analytical range for atropine and scopolamine is 0.01-30 mg/mL. The procedure ensures the simultaneous determination of compounds from other classes of substances.