Journal
JOURNAL OF ANALYTICAL CHEMISTRY
Volume 66, Issue 13, Pages 1298-1306Publisher
PLEIADES PUBLISHING INC
DOI: 10.1134/S1061934811130120
Keywords
Rana lessonae; Fourier-transform ion cyclotrone resonance mass spectrometry; peptide sequencing; disulfide-containing peptides; lessonakinines
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Amphibian skin secretion represents a cerain scientific interest as a source of biologically active natural peptides. In the present research skin peptidome of wide-spread European frog Rana lessonae (Camerano, 1882) was studied for the first time ever. Peptide sequencing was accomplished with Fourier transform ion cyclotron resonance mass spectrometer in collision-induced and electron capture dissociation modes. A portion of amphibian peptides contains intramolecular C-terminal disulfide cycle which obstructs mass spectrometric sequencing. Two methods were utilized to overcome this difficulty: reduction with dithiotreithol followed by thiol group alkylation and oxidation into sulfonic acid groups with performic acid. Integrated approach employed in the present study allowed the identification of 49 peptides (of 6 to 37 amino acid residues), including 19 novel species.
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