Journal
JOURNAL OF ANALYTICAL CHEMISTRY
Volume 66, Issue 9, Pages 807-814Publisher
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S1061934811090164
Keywords
magnetic separation; selective modulators of androgen receptors; peroxisome proliferator-activated delta-receptor agonists; high-performance liquid chromatography; tandem mass spectrometry; doping control
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Funding
- Ministry of Sport, Tourism, and Youth Policy of the Russian Federation [307]
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A new version of extraction is proposed for the extraction of doping substances from urine. It is based on magnetic separation using ferromagnetic microparticles with the surface modified with C(18) groups. A procedure is developed on its basis for the quantification of selective modulators of androgen receptors and peroxisome proliferator-activated delta-receptor agonists using high-performance liquid chromatography coupled with tandem mass spectrometry. Using the proposed approach, the recovery of doping substances from human biological fluids approximates 100%, while the negative effect of the matrix components on the ionization of target compounds is reduced to a minimum. In contrast to solid phase extraction and liquid-liquid extraction, the method of magnetic separation appears to be more rapid and simple. The time of pretreatment for a sample intended for HPLC coupled to tandem mass spectrometry makes 5 min; the detection limit makes 0.25-5 ng/mL.
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